Mission of the Surgical Neuro-Oncology lab
Discovery of cures for patients with brain tumors through ethical scientific inquiry, collaboration, cutting-edge clinical care, education and mentorship. The laboratory focuses on identifying novel immunotherapy approaches to overcome resistance in patients with brain cancer. Current projects include development of vaccines for the treatment of brain tumors and combining immunotherapy with MRI guided laser ablation.
Dr. Rahman’s current interests include providing leading-edge, quality care for patients with brain tumors, teaching and mentoring medical students and residents and performing translational research to discover and implement novel therapies for malignant brain tumors.
Glioblastoma (GBM) is an aggressive form of brain cancer and is uniformly fatal. Standard of care is surgery, radiation, chemotherapy and sometimes tumor-treating fields. Our lab is working on finding novel therapies with the potential to increase survival for patients with GBM.
Hydrogel-CXCL9-mRNA (HCM) vaccine
This is a novel platform developed in our laboratory in collaboration with UF College of Engineering that combines a hydrogel, chemokine and a nanoparticle to deliver tumor mRNA to the immune system. This vaccine results in recruitment of diverse immune cells into the hydrogel which are then educated against the total tumor mRNA. The immune cells then exit the hydrogel and go to the brain tumor to result in a powerful anti-tumor effect. We are currently working on further understanding the mechanism of the efficacy of this vaccine and working on the studies necessary to begin a human clinical trial.
(Left panel) Schematic of the HCM vaccine. The polyethylene glycol (PEG) hydrogel is loaded with mRNA nanoparticles and CXCL9, a chemokine that recruits immune cells. The mRNA nanoparticles are loaded with total tumor RNA (ttRNA). Middle panel) CXCL9 induces migration of various immune cells, including T cells, natural killer (NK) cells, and dendritic cells (DCs), into the HCM vaccine. Right panel) DCs are transfected by mRNA nanoparticles and present tumor associated antigens to T cells, resulting in tumor-specific T cell activation. NK cells secrete IFN-γ which potentiates T cell activation. Activated T cells egress from the HCM vaccine to exert their antitumor effect.
Human GBM cells (blue) being killed by activated T cells (red) in the setting of the HCM vaccine using total tumor mRNA.
Laser interstitial thermotherapy (LITT)
A novel treatment option for the treatment of brain tumors is stereotactic implantation of a laser into the tumor causing thermal ablation of the tumor. This treatment has the additional benefit of opening the blood brain barrier (BBB) and resulting in immune infiltration within the tumor after treatment. We have recently developed a mouse LITT model and are using this to study combinatorial strategies with immunotherapy for the treatment of GBM and brain metastases.
- Immunomodulation with chemotherapy changes response to immunotherapy in glioma
- Optimizing vaccine with enhancing dendritic cell migration
|Aida Karachi DVM, PhD– Post doc and Assistant Research Scientist||Senior Clinical Scientist at Boehringer Ingelheim|
|Farhad Dastmalchi, DVM – Assistant Research Scientist||Senior Scientist Janux Therapeutics|
|Hassan Azari, PhD – Assistant Research Scientist||Associate professor Barry University|
|Rolando Lovaton, MD – Post doc||Practicing neurosurgeon Hospital Nacional Cayetano Heredia Peru|
|Megan Saia, J.D.||Practicing patent law|
|Lisa Kurian||(06/2018-2020) Currently: Matriculated into medical school|
|Sarah Shireen Gul||(06/2013-2020) Currently: Matched in General Surgery|
|Michael Shang – PhD student||07/2016 – 06/2019|
|Rachel Newsome – PhD student||06/2018-01/2019|
|Rolando Espadin||Neurosurgeon in Peru|
|Ashley O’Malley||Matriculated in medical school|
Dr. Rahman’s Lab Team
As a PhD student at the UF College of Medicine, I am studying Medical Physics under the guidance of Dr. Frank J. Bova with the purpose of pursuing a career in clinical work and research. My background studies are in molecular neuroscience and synthetic chemistry with focuses on protocol development for nervous structure imaging and biomimetic compound synthesis, respectively.
My work at the UF Brain Tumor Immunotherapy Program in the Rahman lab has consisted of the development of a novel murine stereotactic laser interstitial thermotherapy (LITT) system and proof of its efficacy, repeatability, and feasibility. In addition to methodology and component development for LITT, I have managed data, inventory, and personnel. My thesis studies are centered around the application of our LITT system for treatment of glioblastoma (GBM) and brain metastases in combination with novel nano-medicine based therapies and immunotherapeutics.
I am a medical student at the University of Florida College of Medicine. I graduated with my B.S. in Chemistry from the University of North Carolina-Chapel Hill in 2020. I worked in Gianpietro Dotti’s lab studying chimeric antigen receptor (CAR) T cell biology for 1 year before beginning medical school and joining the Rahman lab. I received funding from the American Brain Tumor Association to support my work with Dr. Rahman over the 2022 summer. My research interests include immunotherapy for cancer, the brain tumor microenvironment, and translating basic science to clinical practice.
In the Rahman lab, I have studied the effects of our novel HCM vaccine on peripheral immune activation and tumor infiltration. My work focused on the role of natural killer cells on T cell activation near the site of vaccination as well as the effect of CCL4 within the tumor microenvironment on immune infiltration and activation. I have also investigated the feasibility of translating our HCM vaccine into a clinical trial for canine patients with glioblastoma.
I am a third year undergraduate student pursuing a B.S. in biology. Additionally, I am a member of the University of Florida Honors program and Research Scholars program. I have been working in the Rahman lab since May 2021. My current research involves investigating the mechanism of our novel HCM vaccine platform in combination with other immunotherapeutic treatments. I hope to continue conducting research in the future and open my own lab that investigates different immunotherapies for brain tumors.
Outside of my lab work, I push myself to be the best role model I can be for my three younger sisters. I am a big believer in women empowerment– especially in STEM related fields. My main goal in life is to make a lasting impact in healthcare and academia by creating a diverse learning environment where scientific innovation can take place.
I am a second-year undergraduate student obtaining my B.S. in Neuroscience and communication studies. I have been a member of the Rahman lab since January 2022. My work in the Rahman laboratory revolves around optimizing the outcome of Laser Interstitial Thermal Therapy (LITT) in our experimental murine models with Glioblastoma Multiforme (GBM) with the purpose of combining our results with other immunotherapies to obtain translational results.
As I work toward a career in medicine, I strive to conduct research that will advance our knowledge about brain cancer treatment and cures.
Presently, I am a 3rd year undergraduate student in the University of Florida Medical Honors Program working towards completing my B.S. in both Behavioral and Cognitive Neuroscience and Basic Biological and Medical Sciences. I began working in the Rahman Lab in August 2022, where my research has involved investigating the efficacy and underlying mechanism of our novel HCM vaccine. As I transition to become a medical student in August 2023, I intend on conducting research that will allow us to better refine our platform for eventual translation into clinical practice. I hope to pursue a career in academic medicine that will allow for mentorship, scientific contributions to the field of neuro-oncology, and the administration of high-quality holistic care for all.
Anjali Chauhan is a post-doctoral fellow in the Department of Neurosurgery at the University of Florida College of Medicine. Dr. Anjali received her PhD from Jawaharlal Nehru University, India. As a doctoral student, she studied the synthesis and characterisation of iron oxide nanoparticles for the treatment of cancer via magnetic hyperthermia therapy. She also studied the effects of magnetic nanoparticles on the neuronal survival and cognitive functions in the presence of alternating magnetic field. Her research interests include nanotechnology, cancer immunotherapy, role of tumor microenvironment in cancer progression, translational research, molecular pathways involved in cancer immunotherapy.
I am a fourth-year undergraduate student at the University of Florida majoring in health sciences. I have been working in the Rahman lab since September 2021. I first started in the lab doing clinical research with doctor Rahman and one of her residents Carlton Christie on reviewing the efficacy of chest x-rays in a neurological intensive care unit. I am in the process of transitioning into the lab to start working on some of the experiments for the HCM vaccine and immunotherapy treatments.
I will be taking 1-2 gap years upon graduation where I plan to continue working on research and gaining more clinical experience before applying to medical school. My main goal is to continue toward a career in academic medicine and make meaningful contributions to the advancement of science and treatments in neurosurgery.
Principal Investigator Maryam Rahman, MD
Like all human beings, any title given does not fully capture who someone is. At work, Maryam Rahman MD has the title of associate professor in the Department of Neurosurgery at the University of Florida. Working within the Wells Brain Tumor Center at UF, she specializes in the treatment of patients with brain or spinal tumors. Her focus is on novel treatment techniques, including laser interstitial thermotherapy, fluorescence guided surgery, immunotherapy/vaccine therapy and awake cortical mapping during surgery.+
Dr. Rahman received a Bachelor’s Degree in Chemistry from Johns Hopkins University, and attended medical school and completed a neurosurgical residency at UF. During her training she completed a two-year neuro-oncology fellowship. After residency, Dr. Rahman completed a surgical neuro-oncology fellowship at Johns Hopkins University.
“Working under Dr. Rahman’s mentorship has not only taught me how to be a better scientist—but a better human as well.”